Inter-city bus drivers joined the strike in Bekes county, southeastern Hungary and Vas county in the country's west.
The strike, which began at 6 a.m. local time (0500 GMT), was initiated by the Liga trade union's alliance led by Istvan Gasko, head of the Railway Workers' Free Union.
Railway workers were also protesting against the planned privatization of healthcare facilities and new pension rules which are to take effect early next year, Gasko said.
About 10,000 railway workers were involved in the work stoppage on the lines of National Railways MAV and Gyor-Sopron-Ebenfurth Railway.
Some 1,200 to 1,300 passenger trains were being affected by the strike, MAV spokesman Imre Kavalecz said.
Freight train operations were also disrupted and international trains were being held up at the border stations.
As many as 136 organizations have voiced support for the strike against the government's reforms on healthcare and pension systems, Liga spokesman said a day earlier.
A total of 82 Liga members and 20 other organizations would stage strikes on Wednesday, as well as trade unions, civil organizations and some political parties, the spokesman added.
The strike came in the wake of a two-hour warning strike on Nov. 7 and abortive talks between management and the unions on a viable compromise.
